We offer the widest selection high quality, bioactive TNF Superfamily proteins in the life sciences industry. These include an array of ligands and receptors, many of which are exclusive to R&D Systems. Click the buttons below to learn about our new and improved TNF Superfamily proteins.
| New Proteins | ||
| Biotinylated Human TNF-alpha | Human GMP-grade TNF-alpha | Mouse DcTRAIL R1 |
| Mouse APRIL | Mouse CD40 Ligand | Rat CD40 Ligand |
| Cynomolgus Monkey CD40 | Rat CD40 | |
Activity of Biotinylated Recombinant Human TNF-alpha. Recombinant Human TNF-alpha (Catalog # 210-TA; green) and Biotinylated Recombinant Human TNF-alpha (Catalog # BT210; red) both induce cytotoxicity in L929 mouse fibroblast cells in the presence of the metabolic inhibitor, Actinomycin D (Catalog # 1229). The ED50 for this effect is typically 0.025–1.0 ng/mL. |
Mouse APRIL-Induced B Cell Proliferation. Recombinant Mouse April (Catalog # 7907-AP) induces mouse B cell proliferation in the presence of a crosslinking antibody, Anti-Mouse Hemagglutinin/ HA Monoclonal Antibody (Catalog # MAB060). The ED50 for this effect is typically 0.5–3 ng/mL. |
| Improved Proteins | ||
| Human GITR Ligand | Human CD40 Ligand | Mouse Fas Ligand |
CHO-expressed, HA-tagged Recombinant Human GITR Ligand (Catalog # 6987-GL; red) exhibits approximately 10-fold greater activity than our older Sf 21 His-tagged version of Recombinant Human GITR Ligand (Catalog # 694-GL; blue). Activity was assessed by measuring IL-8 secretion by human GITR-transfected HT1080 human fibrosarcoma cells. The ED50 for this effect is typically 4–20 ng/mL. Secreted IL-8 was measured using the Human IL-8 DuoSet® ELISA Development Kit (Catalog # DY208). |
HEK293-expressed, HA-tagged Recombinant Human CD40 Ligand (Catalog # 6420-CL) has 1000-fold greater activity than our older, untagged version of Recombinant Human CD40 Ligand (Catalog # 6245-CL). Activity was assessed by induction of human B cell proliferation in the presence of Recombinant Human IL-4 (Catalog # 204-IL) and a crosslinking, Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060). The ED50 for this effect is approximately 0.5 ng/mL. |
CHO-expressed Recombinant Mouse Fas-Ligand (Catalog # 6128-SA) exhibits approximately 1000-fold greater activity than our older NSO-expressed version of Recombinant Mouse Fas Ligand (Catalog # 526-SA). Activity was assessed by induction of apoptosis of Jurkat human acute T cell leukemia cell in the presence of 2.5 μg/mL of a crosslinking, Anti-Mouse Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060). The ED50 for this effect is typically 1–8 ng/mL. |